产品描述
B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. The ECD of human BTLA shares 42% and 44% aa identity with that of mouse and rat BTLA, respectively. BTLA is also unusual in its interaction with the TNF superfamily member HVEM rather than with B7 family ligands. BTLA is expressed on T cells, B cells, macrophages, dendritic cells, and NK cells. Its expression is low in naïve T cells and increases during antigen-specific induction of anergy. In B cells, BTLA expression is highest in mature naïve cells. BTLA apparently limits T cell numbers, since its deletion results in overproduction of T cells, especially CD8+ memory T cells that are hyper-responsive to TCR crosslinking.
产品性质
别名 |
CD272; BTLA; BTLA1; FLJ16065; MGC129743 |
Uniprot No. |
Q7Z6A9.1 |
表达区间及表达系统 |
Recombinant Biotinylated Human BTLA Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Lys31-Ser150. |
分子量 |
Approximately 16.7 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result. |
纯度 |
> 95% as determined by SDS-PAGE and HPLC. |
活性 |
ELISA Data: Immobilized Human BTLA, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BTLA Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA. SPR Data: Anti-BTLA antibody captured on Protein A chip, can bind Human BTLA, His Tag with an affinity constant of 0.99nM as determined in a SPR assay (Biacore T200). |
内毒素 |
< 1.0 EU per 1μg of the protein by the LAL method. |
制剂 |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
复溶 |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
运输与保存方法
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
产品数据
HB220805
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